Primer

Part:BBa_K5007017

Designed by: Pedro Ribeiro Faria   Group: iGEM23_USP-EEL-Brazil   (2023-10-06)


pRS426 F-primer

Forward primer for amplifying pRS426 and pRS425s around the SmaI restriction site, adding homology regions for cloning part BBa_K5007007 through Gibson Assembly.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 16
    Illegal SpeI site found at 10
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 10
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 16
    Illegal SpeI site found at 10
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 16
    Illegal SpeI site found at 10
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
t_m 65°C